High throughput quantification of short nucleic acid samples by capillary electrophoresis with automated data processing.

ABSTRACT: Analysis of catalytic activity of nucleic acid enzymes is crucial for many applications, ranging from biotechnology to the search for antiviral drugs. Commonly used analytical methods for quantifying DNA and RNA reaction products based on slab-gel electrophoresis are limited in throughput, speed, and accuracy. Here we report the optimization of high throughput methods to separate and quantify short nucleic acid reaction products using DNA sequencing instruments based on capillary electrophoresis with fluorescence detection. These methods afford single base resolution without requiring extensive sample preparation. Additionally, we show that the utility of our system extends to quantifying RNA products. The efficiency and reliability of modern instruments offers a large increase in throughp

SUBMITTER: Dangerfield TL 

PROVIDER: S-EPMC8384658 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature