Systems biology evaluation of refractory Clostridioides difficile infection including multiple failures of fecal microbiota transplantation.

ABSTRACT:

Background

Fecal microbiota transplantation (FMT) aims to cure Clostridioides difficile infection (CDI) through reestablishing a healthy microbiome and restoring colonization resistance. Although often effective after one infusion, patients with continued microbiome disruptions may require multiple FMTs. In this N-of-1 study, we use a systems biology approach to evaluate CDI in a patient receiving chronic suppressive antibiotics with four failed FMTs over two years.

Methods

Seven stool samples were obtained between 2016-18 while the patient underwent five FMTs. Stool samples were cultured for C. difficile and underwent microbial characterization and functional gene analysis using shotgun metagenomics. C. difficile isolates were characterized through ribotyping, whole genome

SUBMITTER: Gonzales-Luna AJ 

PROVIDER: S-EPMC8384661 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature