Upregulation of Local Hepcidin Contributes to Iron Accumulation in Alzheimer's Disease Brains.

ABSTRACT:

Background

Accumulation of iron is a consistent feature of Alzheimer's disease (AD) brains. The underlying cause, however, remains debatable.

Objective

To explore whether local hepcidin synthesized by brain cells contributes to iron accumulation in AD brains.

Methods

Brain tissue from the cingulate cortex of 33 cases of AD pre-assigned to Braak stage I-VI, 6 cases of non-dementia, and 15 cases of non-AD dementia were analyzed for transcriptional upregulation of hepcidin by RT-qPCR and RT-PCR. Change in the expression of ferritin, ferroportin (Fpn), microglial activation marker Iba1, IL-6, and TGFβ2 was determined by western blotting. Total tissue iron was determined by colorimetry.

Results

Significant transcriptional upregulation of hepcidin was observed in Bra

SUBMITTER: Chaudhary S 

PROVIDER: S-EPMC8384696 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature