Ontology highlight
ABSTRACT:
SUBMITTER: Corpas-Lopez V
PROVIDER: S-EPMC8384900 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Corpas-Lopez Victoriano V Wyllie Susan S
STAR protocols 20210818 3
Here, we detail our optimized protocol for the identification of drug targets in <i>Leishmania donovani</i> using thermal proteome profiling. This approach is based on the principle that binding of a drug to its protein target can significantly alter the thermal stability of that protein. By monitoring changes in the thermal stability of proteins within drug-treated and untreated cell lysates, using mass spectrometry combined with tandem mass tag labeling, putative targets of the drug can be ide ...[more]