Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors.

ABSTRACT: The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax6⁺ granule cells and Calbindin⁺ Purkinje cells from common Sox2⁺ embryonic cerebellar progenitors. We describe the procedure for dissecting mouse cerebellar anlage, cell seeding, and tamoxifen-induced labeling of progenitor cells, followed by time-lapse video recording of clonal expansion and neuronal differentiation. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

SUBMITTER: Zhang T 

PROVIDER: S-EPMC8384916 | biostudies-literature |

REPOSITORIES: biostudies-literature