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Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors.

ABSTRACT: The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax6⁺ granule cells and Calbindin⁺ Purkinje cells from common Sox2⁺ embryonic cerebellar progenitors. We describe the procedure for dissecting mouse cerebellar anlage, cell seeding, and tamoxifen-induced labeling of progenitor cells, followed by time-lapse video recording of clonal expansion and neuronal differentiation. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021).

SUBMITTER: Zhang T

PROVIDER: S-EPMC8384916 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Induction of granule and Purkinje cells from primary cultured mouse cerebellar progenitors.

Zhang Tingting T Liu Tengyuan T Hassan Bassem A BA

STAR protocols 20210818 3

The architecturally stereotypical structure of cerebellum is ideal for investigating the generation of neuronal diversity, but in vitro models for assessing early cerebellar progenitor differentiation were lacking. Here, we report a detailed protocol for long-term in vitro generation of Pax6+ granule cells and Calbindin+ Purkinje cells from common Sox2+ embryonic cerebellar progenitors. We describe the procedure for dissecting mouse cerebellar anlag ...[more]

PMID: 34467229

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