Single cell functional genomics reveals plasticity of subcutaneous white adipose tissue (WAT) during early postnatal development.

ABSTRACT:

Objective

The current study addresses the cellular complexity and plasticity of subcutaneous (inguinal) white adipose tissue (iWAT) in mice during the critical periods of perinatal growth and establishment.

Methods

We performed a large-scale single cell transcriptomic (scRNA-seq) and epigenomic (snATAC-seq) characterization of cellular subtypes (adipose stromal cells (ASC) and adipocyte nuclei) during inguinal WAT (subcutaneous; iWAT) development in mice, capturing the early postnatal period (postnatal days (PND) 06 and 18) through adulthood (PND56).

Results

Perinatal and adult iWAT contain 3 major ASC subtypes that can be independently identified by RNA expression profiles and DNA transposase accessibility. Furthermore, the transcriptomes and enhancer landscapes of b

SUBMITTER: Rondini EA 

PROVIDER: S-EPMC8385178 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature