Soluble trivalent engagers redirect cytolytic T cell activity toward tumor endothelial marker 1.

ABSTRACT: Tumor endothelial marker 1 (TEM1) is an emerging cancer target with a unique dual expression profile. First, TEM1 is expressed in the stroma and neo-vasculature of many human carcinomas but is largely absent from healthy adult tissues. Second, TEM1 is expressed by tumor cells of mesenchymal origin, notably sarcoma. Here, we present two fully human anti-TEM1 single-chain variable fragment (scFv) reagents, namely, 1C1m and 7G22, that recognize distinct regions of the extracellular domain and possess substantially different affinities. In contrast to other, well-described anti-TEM1 binders, these fragments confer cytolytic activity when expressed as 2^nd generation chimeric antigen receptors (CARs). Moreover, both molecules selectively redirect human T cell effector functions toward

SUBMITTER: Fierle JK 

PROVIDER: S-EPMC8385295 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature