Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology.

ABSTRACT:

Background

Genomes can be sequenced with relative ease, but ascribing gene function remains a major challenge. Genetically tractable model systems are crucial to meet this challenge. One powerful model is the social amoeba Dictyostelium discoideum, a eukaryotic microbe widely used to study diverse questions in the cell, developmental and evolutionary biology.

Results

We describe REMI-seq, an adaptation of Tn-seq, which allows high throughput, en masse, and quantitative identification of the genomic site of insertion of a drug resistance marker after restriction enzyme-mediated integration. We use REMI-seq to develop tools which greatly enhance the efficiency with which the sequence, transcriptome or proteome variation can be linked to phenotype in D. discoideum. These compri

SUBMITTER: Gruenheit N 

PROVIDER: S-EPMC8386026 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature