An economical and highly adaptable optogenetics system for individual and population-level manipulation of Caenorhabditis elegans.

ABSTRACT:

Background

Optogenetics allows the experimental manipulation of excitable cells by a light stimulus without the need for technically challenging and invasive procedures. The high degree of spatial, temporal, and intensity control that can be achieved with a light stimulus, combined with cell type-specific expression of light-sensitive ion channels, enables highly specific and precise stimulation of excitable cells. Optogenetic tools have therefore revolutionized the study of neuronal circuits in a number of models, including Caenorhabditis elegans. Despite the existence of several optogenetic systems that allow spatial and temporal photoactivation of light-sensitive actuators in C. elegans, their high costs and low flexibility have limited wide access to optogenetics. Here, we deve

SUBMITTER: Koopman M 

PROVIDER: S-EPMC8386059 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature