Absent from DNA and protein: genomic characterization of nullomers and nullpeptides across functional categories and evolution.

ABSTRACT: Nullomers and nullpeptides are short DNA or amino acid sequences that are absent from a genome or proteome, respectively. One potential cause for their absence could be their having a detrimental impact on an organism. RESULTS: Here, we identify all possible nullomers and nullpeptides in the genomes and proteomes of thirty eukaryotes and demonstrate that a significant proportion of these sequences are under negative selection. We also identify nullomers that are unique to specific functional categories: coding sequences, exons, introns, 5'UTR, 3'UTR, promoters, and show that coding sequence and promoter nullomers are most likely to be selected against. By analyzing all protein sequences across the tree of life, we further identify 36,081 peptides up to six amino acids in length that do not

SUBMITTER: Georgakopoulos-Soares I 

PROVIDER: S-EPMC8386077 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature