A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applications.

ABSTRACT:

Background aims

Next-generation immune cell therapy products will require complex modifications using engineering technologies that can maintain high levels of cell functionality. Non-viral engineering methods have the potential to address limitations associated with viral vectors. However, while electroporation is the most widely used non-viral modality, concerns about its effects on cell functionality have led to the exploration of alternative approaches. Here the authors have examined the suitability of the Solupore non-viral delivery system for engineering primary human T cells for cell therapy applications.

Methods

The Solupore system was used to deliver messenger RNA (mRNA) and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein

SUBMITTER: Kavanagh H 

PROVIDER: S-EPMC8386197 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature