A Novel SXT/R391 Integrative and Conjugative Element Carries Two Copies of the bla_NDM-1 Gene in Proteus mirabilis.

ABSTRACT: The rapid spread of the bla_NDM-1 gene is a major public health concern. Here, we describe the multidrug-resistant Proteus mirabilis strain XH1653, which contains a novel SXT/R391 integrative and conjugative element (ICE), harboring two tandem copies of bla_NDM-1 and 21 other resistance genes. XH1653 was resistant to all antibiotics tested, apart from aztreonam. Whole-genome data revealed that two copies of bla_NDM-1 embedded in the ISCR1 element are located in HS4 of the novel ICE, which we named ICEPmiChnXH1653. A circular intermediate of ICEPmiChnXH1653 was detected by PCR, and conjugation experiments showed that the ICE can be transferred to the Escherichia coli strain EC600 with frequencies of 1.5 × 10^-7

SUBMITTER: He J 

PROVIDER: S-EPMC8386438 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature