CCL2 associated with CD38 expression during ex vivo expansion in human cord blood-derived hematopoietic stem cells.

ABSTRACT: To date, different experimental strategies have been developed for the ex vivo expansion of human hematopoietic stem cells (HSCs) for clinical applications. However, differences in the genomic function of expanded HSCs under different culture systems remain unclear. In this study, we compared the gene expression profiles of HSCs in ex vivo expanded serum (10% FBS, fetal bovine serum) and serum-free culture systems and analyzed the molecular functions of differentially expressed genes using microarray chips. We identified 839 differentially expressed genes between the two culture systems. These genes were enriched in the TNF -regulated inflammatory pathway in an FBS culture system. In addition, the mRNA expression of CCL2 (C-C motif chemokine ligand 2), TNF (tumor necrosis fac

SUBMITTER: Yao CL 

PROVIDER: S-EPMC8386547 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature