A chemical genomics-aggrephagy integrated method studying functional analysis of autophagy inducers.

ABSTRACT: Macroautophagy/autophagy plays a critical role in the pathogenesis of various human diseases including neurodegenerative disorders such as Parkinson disease (PD) and Huntington disease (HD). Chemical autophagy inducers are expected to serve as disease-modifying agents by eliminating cytotoxic/damaged proteins. Although many autophagy inducers have been identified, their precise molecular mechanisms are not fully understood because of the complicated crosstalk among signaling pathways. To address this issue, we performed several chemical genomic analyses enabling us to comprehend the dominancy among the autophagy-associated pathways followed by an aggresome-clearance assay. In a first step, more than 400 target-established small molecules were assessed for their ability to activate autophag

SUBMITTER: Kataura T 

PROVIDER: S-EPMC8386610 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature