Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B.

ABSTRACT:

Background

Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic 'mucolytic' activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology.

Methods

The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometr

SUBMITTER: Robertsson C 

PROVIDER: S-EPMC8386731 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature