Improved epitope resolution of the prefusion trimer-specific antibody AM14 bound to the RSV F glycoprotein.

ABSTRACT: Respiratory syncytial virus (RSV) is the most common cause of acute lower respiratory tract infections resulting in medical intervention and hospitalizations during infancy and early childhood, and vaccination against RSV remains a public health priority. The RSV F glycoprotein is a major target of neutralizing antibodies, and the prefusion stabilized form of F (DS-Cav1) is under investigation as a vaccine antigen. AM14 is a human monoclonal antibody with the exclusive capacity of binding an epitope on prefusion F (PreF), which spans two F protomers. The quality of recognizing a trimer-specific epitope makes AM14 valuable for probing PreF-based immunogen conformation and functionality during vaccine production. Currently, only a low-resolution (5.5 Å) X-ray structure is available of the Pr

SUBMITTER: Harshbarger W 

PROVIDER: S-EPMC8386734 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature