Targeting hepatocyte growth factor in epithelial-stromal interactions in an in vitro experimental model of human periodontitis.

ABSTRACT: Periodontitis is a chronic inflammatory disease leading to progressive connective tissue degradation and loss of the tooth-supporting bone. Clinical and experimental studies suggest that hepatocyte growth factor (HGF) is involved in the dysregulated fibroblast-epithelial cell interactions in periodontitis. The aim of this study was to explore effects of HGF to impact fibroblast-induced collagen degradation. A patient-derived experimental cell culture model of periodontitis was applied. Primary human epithelial cells and fibroblasts isolated from periodontitis-affected gingiva were co-cultured in a three-dimensional collagen gel. The effects of HGF neutralizing antibody on collagen gel degradation were tested and transcriptome analyses were performed. HGF neutralizing antibody attenuated co

SUBMITTER: Yamaguchi Y 

PROVIDER: S-EPMC8387255 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature