Enhanced CD4⁺ and CD8⁺ T cell infiltrate within convex hull defined pancreatic islet borders as autoimmune diabetes progresses.

ABSTRACT: The notion that T cell insulitis increases as type 1 diabetes (T1D) develops is unsurprising, however, the quantitative analysis of CD4⁺ and CD8⁺ T cells within the islet mass is complex and limited with standard approaches. Optical microscopy is an important and widely used method to evaluate immune cell infiltration into pancreatic islets of Langerhans for the study of disease progression or therapeutic efficacy in murine T1D. However, the accuracy of this approach is often limited by subjective and potentially biased qualitative assessment of immune cell subsets. In addition, attempts at quantitative measurements require significant time for manual analysis and often involve sophisticated and expensive imaging software. In this study, we developed and illustrate he

SUBMITTER: Dwyer AJ 

PROVIDER: S-EPMC8387412 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature