Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri.

ABSTRACT: Over the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. Propagation of laboratory inbred strains of Nothobranchius furzeri, such as GRZ, however, can pose challenges due to the short window of fertility, the efforts and space requirements involved in continuous strain maintenance, and the risks of further inbreeding. The current method for long term strain preservation relies on arrest of embryos in diapause. To create an alternative for long term maintenance, we developed a robust protocol to cryopreserve and revive sperm for in vitro fertilization (IVF). We tested a variety of extender and activator buffers for sperm IVF, as well as cryoprotectants to achieve practical long-ter

SUBMITTER: Dolfi L 

PROVIDER: S-EPMC8387425 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature