Differential inflammatory responses of the native left and right ventricle associated with donor heart preservation.

ABSTRACT:

Background

Dysfunction and inflammation of hearts subjected to cold ischemic preservation may differ between left and right ventricles, suggesting distinct strategies for amelioration.

Methods and results

Explanted murine hearts subjected to cold ischemia for 0, 4, or 8 h in preservation solution were assessed for function during 60 min of warm perfusion and then analyzed for cell death and inflammation by immunohistochemistry and western blotting and total RNA sequencing. Increased cold ischemic times led to greater left ventricle (LV) dysfunction compared to right ventricle (RV). The LV experienced greater cell death assessed by TUNEL⁺ cells and cleaved caspase-3 expression (n = 4). While IL-6 protein levels were upregulated in both LV and RV, IL-1β, TNFα, IL-10

SUBMITTER: Lei I 

PROVIDER: S-EPMC8387788 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature