Assessing the Resolution of Methyltransferase-Mediated DNA Optical Mapping.

ABSTRACT: Interest in the human microbiome is growing and has been, for the past decade, leading to new insights into disease etiology and general human biology. Stimulated by these advances and in a parallel trend, new DNA sequencing platforms have been developed, radically expanding the possibilities in microbiome research. While DNA sequencing plays a pivotal role in this field, there are some technological hurdles that are yet to be overcome. Targeting of the 16S rRNA gene with amplicon sequencing, for instance, is frequently used for sample composition profiling due to its short sample-to-result time and low cost, which counterbalance its low resolution (genus to species level). On the other hand, more comprehensive methods, namely, whole-genome sequencing (WGS) and shallow shotgun sequencing,

SUBMITTER: D'Huys L 

PROVIDER: S-EPMC8387989 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature