Release of Iron-Loaded Ferritin in Sodium Iodate-Induced Model of Age Related Macular Degeneration: An In-Vitro and In-Vivo Study.

ABSTRACT: To evaluate the role of iron in sodium iodate (NaIO₃)-induced model of age-related macular degeneration (AMD) in ARPE-19 cells in-vitro and in mouse models in-vivo. ARPE-19 cells, a human retinal pigment epithelial cell line, was exposed to 10 mM NaIO₃ for 24 h, and the expression and localization of major iron modulating proteins was evaluated by Western blotting (WB) and immunostaining. Synthesis and maturation of cathepsin-D (cat-D), a lysosomal enzyme, was evaluated by quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR) and WB, respectively. For in-vivo studies, C57BL/6 mice were injected with 40 mg/kg mouse body weight of NaIO₃ intraperitoneally, and their retina was evaluated after 3 weeks as above. NaIO₃ induced a 10-fol

SUBMITTER: Ashok A 

PROVIDER: S-EPMC8389213 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature