Step-by-step guide to efficient subtomogram averaging of virus-like particles with Dynamo.

ABSTRACT: Subtomogram averaging (STA) is a powerful image processing technique in electron tomography used to determine the 3D structure of macromolecular complexes in their native environments. It is a fast growing technique with increasing importance in structural biology. The computational aspect of STA is very complex and depends on a large number of variables. We noticed a lack of detailed guides for STA processing. Also, current publications in this field often lack a documentation that is practical enough to reproduce the results with reasonable effort, which is necessary for the scientific community to grow. We therefore provide a complete, detailed, and fully reproducible processing protocol that covers all aspects of particle picking and particle alignment in STA. The command line-based wo

SUBMITTER: Scaramuzza S 

PROVIDER: S-EPMC8389376 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature