Translation of ABCE1 Is Tightly Regulated by Upstream Open Reading Frames in Human Colorectal Cells.

ABSTRACT: ATP-binding cassette subfamily E member 1 (ABCE1) belongs to the ABC protein family of transporters; however, it does not behave as a drug transporter. Instead, ABCE1 actively participates in different stages of translation and is also associated with oncogenic functions. Ribosome profiling analysis in colorectal cancer cells has revealed a high ribosome occupancy in the human ABCE1 mRNA 5'-leader sequence, indicating the presence of translatable upstream open reading frames (uORFs). These cis-acting translational regulatory elements usually act as repressors of translation of the main coding sequence. In the present study, we dissect the regulatory function of the five AUG and five non-AUG uORFs identified in the human ABCE1 mRNA 5'-leader sequence. We show that the e

SUBMITTER: Silva J 

PROVIDER: S-EPMC8389594 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature