Sequencing and Chromosome-Scale Assembly of Plant Genomes, Brassica rapa as a Use Case.

ABSTRACT: With the rise of long-read sequencers and long-range technologies, delivering high-quality plant genome assemblies is no longer reserved to large consortia. Not only sequencing techniques, but also computer algorithms have reached a point where the reconstruction of assemblies at the chromosome scale is now feasible at the laboratory scale. Current technologies, in particular long-range technologies, are numerous, and selecting the most promising one for the genome of interest is crucial to obtain optimal results. In this study, we resequenced the genome of the yellow sarson, Brassica rapa cv. Z1, using the Oxford Nanopore PromethION sequencer and assembled the sequenced data using current assemblers. To reconstruct complete chromosomes, we used and compared three long-range scaffol

SUBMITTER: Istace B 

PROVIDER: S-EPMC8389630 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature