The PilB-PilZ-FimX regulatory complex of the Type IV pilus from Xanthomonas citri.

ABSTRACT: Type IV pili (T4P) are thin and flexible filaments found on the surface of a wide range of Gram-negative bacteria that undergo cycles of extension and retraction and participate in a variety of important functions related to lifestyle, defense and pathogenesis. During pilus extensions, the PilB ATPase energizes the polymerization of pilin monomers from the inner membrane. In Xanthomonas citri, two cytosolic proteins, PilZ and the c-di-GMP receptor FimX, are involved in the regulation of T4P biogenesis through interactions with PilB. In vivo fluorescence microscopy studies show that PilB, PilZ and FimX all colocalize to the leading poles of X. citri cells during twitching motility and that this colocalization is dependent on the presence of all three proteins. We demonstrate that full-lengt

SUBMITTER: Llontop EE 

PROVIDER: S-EPMC8389850 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature