Immune Characters and Plasticity of the Sentinel Lymph Node in Colorectal Cancer Patients.

ABSTRACT:

Purpose

This study is aimed at immunologically characterizing sentinel lymph nodes (SNs) in colorectal cancer (CRC) patients and identifying changes in immunological phenotype and function of SNs isolated from the tumor immunosuppressive microenvironment.

Methods

A total of 53 pairs of matched SNs and non-SNs (NSNs) were collected by using a lymph node tracer dye. Flow cytometry was performed to detect the immunophenotype of T cells as well as the expression of activation and inhibitory markers. Differential expression and distribution of characteristic immune cell markers were analyzed by multiplex immunohistochemistry (mIHC). Transcriptomics analysis was conducted to compare the differences in the expression of immune-related genes among lymph nodes. The ex vivo cul

SUBMITTER: Li X 

PROVIDER: S-EPMC8390165 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature