Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor.

ABSTRACT: The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affectin

SUBMITTER: Moya-Torres A 

PROVIDER: S-EPMC8390410 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature