Topically delivered 22 nt siRNAs enhance RNAi silencing of endogenous genes in two species.

ABSTRACT:

Main conclusion

22 nt siRNAs applied to leaves induce production of transitive sRNAs for targeted genes and can enhance local silencing. Systemic silencing was only observed for a GFP transgene. RNA interference (RNAi) is a gene silencing mechanism important in regulating gene expression during plant development, response to the environment and defense. Better understanding of the molecular mechanisms of this pathway may lead to future strategies to improve crop traits of value. An abrasion method to deliver siRNAs into leaf cells of intact plants was used to investigate the activities of 21 and 22 nt siRNAs in silencing genes in Nicotiana benthamiana and Amaranthus cruentus. We confirmed that both 21 and 22 nt siRNAs were able to silence a green fluorescent protein (GFP) transgene

SUBMITTER: Hendrix B 

PROVIDER: S-EPMC8390415 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature