Genomic skimming and nanopore sequencing uncover cryptic hybridization in one of world's most threatened primates.

ABSTRACT: The Brazilian buffy-tufted-ear marmoset (Callithrix aurita), one of the world's most endangered primates, is threatened by anthropogenic hybridization with exotic, invasive marmoset species. As there are few genetic data available for C. aurita, we developed a PCR-free protocol with minimal technical requirements to rapidly generate genomic data with genomic skimming and portable nanopore sequencing. With this direct DNA sequencing approach, we successfully determined the complete mitogenome of a marmoset that we initially identified as C. aurita. The obtained nanopore-assembled sequence was highly concordant with a Sanger sequenced version of the same mitogenome. Phylogenetic analyses unexpectedly revealed that our specimen was a cryptic hybrid, with a C. aurita phenotype and C. penicilla

SUBMITTER: Malukiewicz J 

PROVIDER: S-EPMC8390465 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature