Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222.

ABSTRACT: Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N₂O/N₂ ratios compared to alkaline soils. In this work, impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with nitrate as sole energy and nitrogen source under anaerobic conditions at pH ranging from 6.5 to 7.5. Quantitative proteomic analysis revealed that the highest difference in protein representation was observed when the proteome at pH 6.5 was compared to the reference proteome at pH 7.2. However, this difference in the extracellular pH was not

SUBMITTER: Olaya-Abril A 

PROVIDER: S-EPMC8390676 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature