Efficient intracellular delivery of proteins by a multifunctional chimaeric peptide in vitro and in vivo.

ABSTRACT: Protein delivery with cell-penetrating peptide is opening up the possibility of using targets inside cells for therapeutic or biological applications; however, cell-penetrating peptide-mediated protein delivery commonly suffers from ineffective endosomal escape and low tolerance in serum, thereby limiting in vivo efficacy. Here, we present an intracellular protein delivery system consisting of four modules in series: cell-penetrating peptide, pH-dependent membrane active peptide, endosome-specific protease sites and a leucine zipper. This system exhibits enhanced delivery efficiency and serum tolerance, depending on proteolytic cleavage-facilitated endosomal escape and leucine zipper-based dimerisation. Intravenous injection of protein phosphatase 1B fused with this system successfully sup

SUBMITTER: Yu S 

PROVIDER: S-EPMC8390694 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature