Self-assembling human heart organoids for the modeling of cardiac development and congenital heart disease.

ABSTRACT: Congenital heart defects constitute the most common human birth defect, however understanding of how these disorders originate is limited by our ability to model the human heart accurately in vitro. Here we report a method to generate developmentally relevant human heart organoids by self-assembly using human pluripotent stem cells. Our procedure is fully defined, efficient, reproducible, and compatible with high-content approaches. Organoids are generated through a three-step Wnt signaling modulation strategy using chemical inhibitors and growth factors. Heart organoids are comparable to age-matched human fetal cardiac tissues at the transcriptomic, structural, and cellular level. They develop sophisticated internal chambers with well-organized multi-lineage cardiac cell types, recapitula

SUBMITTER: Lewis-Israeli YR 

PROVIDER: S-EPMC8390749 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature