EPA and DHA differentially modulate membrane elasticity in the presence of cholesterol.

ABSTRACT: Polyunsaturated fatty acids (PUFAs) modify the activity of a wide range of membrane proteins and are increasingly hypothesized to modulate protein activity by indirectly altering membrane physical properties. Among the various physical properties affected by PUFAs, the membrane area expansion modulus (K_a), which measures membrane strain in response to applied force, is expected to be a significant controller of channel activity. Yet, the impact of PUFAs on membrane K_a has not been measured previously. Through a series of micropipette aspiration studies, we measured the apparent K_a (K_app) of phospholipid model membranes containing nonesterified fatty acids. First, we measured membrane K_app as a function of the location of the unsaturate

SUBMITTER: Jacobs ML 

PROVIDER: S-EPMC8390804 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature