Nonthermal excitation effects mediated by sub-terahertz radiation on hydrogen exchange in ubiquitin.

ABSTRACT: Water dynamics in the hydration layers of biomolecules play crucial roles in a wide range of biological functions. A hydrated protein contains multiple components of diffusional and vibrational dynamics of water and protein, which may be coupled at ∼0.1-THz frequency (10-ps timescale) at room temperature. However, the microscopic description of biomolecular functions based on various modes of protein-water-coupled motions remains elusive. A novel approach for perturbing the hydration dynamics in the subterahertz frequency range and probing them at the atomic level is therefore warranted. In this study, we investigated the effect of klystron-based, intense 0.1-THz excitation on the slow dynamics of ubiquitin using NMR-based measurements of hydrogen-deuterium exchange. We demonstrated that t

SUBMITTER: Tokunaga Y 

PROVIDER: S-EPMC8390810 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature