AutoSmarTrace: Automated chain tracing and flexibility analysis of biological filaments.

ABSTRACT: Single-molecule imaging is widely used to determine statistical distributions of molecular properties. One such characteristic is the bending flexibility of biological filaments, which can be parameterized via the persistence length. Quantitative extraction of persistence length from images of individual filaments requires both the ability to trace the backbone of the chains in the images and sufficient chain statistics to accurately assess the persistence length. Chain tracing can be a tedious task, performed manually or using algorithms that require user input and/or supervision. Such interventions have the potential to introduce user-dependent bias into the chain selection and tracing. Here, we introduce a fully automated algorithm for chain tracing and determination of persistence leng

SUBMITTER: Schneider M 

PROVIDER: S-EPMC8390895 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature