Modeling reveals cortical dynein-dependent fluctuations in bipolar spindle length.

ABSTRACT: Proper formation and maintenance of the mitotic spindle is required for faithful cell division. Although much work has been done to understand the roles of the key molecular components of the mitotic spindle, identifying the consequences of force perturbations in the spindle remains a challenge. We develop a computational framework accounting for the minimal force requirements of mitotic progression. To reflect early spindle formation, we model microtubule dynamics and interactions with major force-generating motors, excluding chromosome interactions that dominate later in mitosis. We directly integrate our experimental data to define and validate the model. We then use simulations to analyze individual force components over time and their relationship to spindle dynamics, making it distin

SUBMITTER: Mercadante DL 

PROVIDER: S-EPMC8390970 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature