Functionalized supported membranes for quantifying adhesion of P. falciparum-infected erythrocytes.

ABSTRACT: The pathology of Plasmodium falciparum malaria is largely defined by the cytoadhesion of infected erythrocytes to the microvascular endothelial lining. The complexity of the endothelial surface and the large range of interactions available for the infected erythrocyte via parasite-encoded adhesins make analysis of critical contributions during cytoadherence challenging to define. Here, we have explored supported membranes functionalized with two important adhesion receptors, ICAM1 or CD36, as a quantitative biomimetic surface to help understand the processes involved in cytoadherence. Parasitized erythrocytes bound to the receptor-functionalized membranes with high efficiency and selectivity under both static and flow conditions, with infected wild-type erythrocytes displaying a higher bin

SUBMITTER: Frohlich B 

PROVIDER: S-EPMC8391081 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature