Stability Assessment of Four Chimeric Proteins for Human Chagas Disease Immunodiagnosis.

ABSTRACT: The performance of an immunoassay relies on antigen-antibody interaction; hence, antigen chemical stability and structural integrity are paramount for an efficient assay. We conducted a functional, thermostability and long-term stability analysis of different chimeric antigens (IBMP), in order to assess effects of adverse conditions on four antigens employed in ELISA to diagnose Chagas disease. ELISA-based immunoassays have served as a model for biosensors development, as both assess molecular interactions. To evaluate thermostability, samples were heated and cooled to verify heat-induced denaturation reversibility. In relation to storage stability, the antigens were analyzed at 25 °C at different moments. Long-term stability tests were performed using eight sets of microplates sensitized.

SUBMITTER: Celedon PAF 

PROVIDER: S-EPMC8391164 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature