Generation, Characterization, and Application of Inducible Proliferative Adult Human Epicardium-Derived Cells.

ABSTRACT:

Rationale

In recent decades, the great potential of human epicardium-derived cells (EPDCs) as an endogenous cell source for cardiac regeneration has been recognized. The limited availability and low proliferation capacity of primary human EPDCs and phenotypic differences between EPDCs obtained from different individuals hampers their reproducible use for experimental studies.

Aim

To generate and characterize inducible proliferative adult human EPDCs for use in fundamental and applied research.

Methods and results

Inducible proliferation of human EPDCs was achieved by doxycycline-controlled expression of simian virus 40 large T antigen (LT) with a repressor-based lentiviral Tet-On system. In the presence of doxycycline, these inducible EPDCs (iEPDCs) displayed high and

SUBMITTER: Ge Y 

PROVIDER: S-EPMC8391799 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature