MitoWave: Spatiotemporal analysis of mitochondrial membrane potential fluctuations during I/R.

ABSTRACT: Mitochondria exhibit unstable inner membrane potentials (ΔΨ_m) when subjected to stress, such as during ischemia/reperfusion (I/R). Understanding the mechanism of ΔΨ_m instability involves characterizing and quantifying this phenomenon in an unbiased and reproducible manner. Here, we describe a simple analytical workflow called "MitoWave" that combines wavelet transform methods and image segmentation to unravel dynamic ΔΨ_m changes in the cardiac mitochondrial network during I/R. In vitro ischemia was affected by placing a glass coverslip on a monolayer of neonatal mouse ventricular myocytes for 1 h and removing the coverslip to allow for reperfusion, revealing complex oscillatory ΔΨ_m. MitoWave analysis was then used to identify individual mitochond

SUBMITTER: Ashok D 

PROVIDER: S-EPMC8392080 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature