High Throughput miRNA Screening Identifies miR-574-3p Hyperproductive Effect in CHO Cells.

ABSTRACT: CHO is the cell line of choice for the manufacturing of many complex biotherapeutics. The constant upgrading of cell productivity is needed to meet the growing demand for these life-saving drugs. Manipulation of small non-coding RNAs-miRNAs-is a good alternative to a single gene knockdown approach due to their post-transcriptional regulation of entire cellular pathways without posing translational burden to the production cell. In this study, we performed a high-throughput screening of 2042-human miRNAs and identified several candidates able to increase cell-specific and overall production of Erythropoietin and Etanercept in CHO cells. Some of these human miRNAs have not been found in Chinese hamster cells and yet were still effective in them. We identified miR-574-3p as being able, when o

SUBMITTER: Svab Z 

PROVIDER: S-EPMC8392531 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature