Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease.

ABSTRACT: The extracellular matrix (ECM) is a key interface between the cerebrovasculature and adjacent brain tissues. Deregulation of the ECM contributes to a broad range of neurological disorders. However, despite this importance, our understanding of the ECM composition remains very limited mainly due to difficulties in its isolation. To address this, we developed an approach to extract the cerebrovascular ECM from mouse and human post-mortem normal brain tissues. We then used mass spectrometry with off-line high-pH reversed-phase fractionation to increase the protein detection. This identified more than 1000 proteins in the ECM-enriched fraction, with > 66% of the proteins being common between the species. We report 147 core ECM proteins of the human brain vascular matrisome, including collagens

SUBMITTER: Pokhilko A 

PROVIDER: S-EPMC8392779 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature