Real-Time Analysis of Oxygen Gradient in Oocyte Respiration Using a High-Density Microelectrode Array.

ABSTRACT: Physiological events related to oxygen concentration gradients provide valuable information to determine the state of metabolizing biological cells. The existing oxygen sensing methods (i.e., optical photoluminescence, magnetic resonance, and scanning electrochemical) are well-established and optimized for existing in vitro analyses. However, such methods also present various limitations in resolution, real-time sensing performance, complexity, and costs. An electrochemical imaging system with an integrated microelectrode array (MEA) would offer attractive means of measuring oxygen consumption rate (OCR) based on the cell's two-dimensional (2D) oxygen concentration gradient. This paper presents an application of an electrochemical sensor platform with a custom-designed complementary-metal-

SUBMITTER: Tedjo W 

PROVIDER: S-EPMC8393405 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature