Genome-wide profiling of alternative splicing in glioblastoma and their clinical value.

ABSTRACT:

Background

Alternative splicing (AS), one of the main post-transcriptional biological regulation mechanisms, plays a key role in the progression of glioblastoma (GBM). Systematic AS profiling in GBM is limited and urgently needed.

Methods

TCGA SpliceSeq data and the corresponding clinical data were downloaded from the TCGA data portal. Survival-related AS events were identified through Kaplan-Meier survival analysis and univariate Cox analysis. Then, splicing correlation network was constructed based on these AS events and associated splicing factors. LASSO regression followed by multivariate Cox analysis was performed to validate independent AS biomarkers and to construct a risk prediction model. Enrichment analysis was subsequently conducted to explore potential signaling

SUBMITTER: Li Y 

PROVIDER: S-EPMC8393481 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature