Establishment of Pancreatobiliary Cancer Zebrafish Avatars for Chemotherapy Screening.

ABSTRACT: Cancers of the pancreas and biliary tree remain one of the most aggressive oncological malignancies, with most patients relying on systemic chemotherapy. However, effective biomarkers to predict the best therapy option for each patient are still lacking. In this context, an assay able to evaluate individual responses prior to treatment would be of great value for clinical decisions. Here we aimed to develop such a model using zebrafish xenografts to directly challenge pancreatic cancer cells to the available chemotherapies. Zebrafish xenografts were generated from a Panc-1 cell line to optimize the pancreatic setting. Pancreatic surgical resected samples, without in vitro expansion, were used to establish zebrafish patient-derived xenografts (zAvatars). Upon chemotherapy exposure, zAvatars

SUBMITTER: Tavares Barroso M 

PROVIDER: S-EPMC8393525 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature