Human reference gut microbiome catalog including newly assembled genomes from under-represented Asian metagenomes.

ABSTRACT:

Background

Metagenome sampling bias for geographical location and lifestyle is partially responsible for the incomplete catalog of reference genomes of gut microbial species. Thus, genome assembly from currently under-represented populations may effectively expand the reference gut microbiome and improve taxonomic and functional profiling.

Methods

We assembled genomes using public whole-metagenomic shotgun sequencing (WMS) data for 110 and 645 fecal samples from India and Japan, respectively. In addition, we assembled genomes from newly generated WMS data for 90 fecal samples collected from Korea. Expecting genome assembly for low-abundance species may require a much deeper sequencing than that usually employed, so we performed ultra-deep WMS (> 30 Gbp or > 100 million read

SUBMITTER: Kim CY 

PROVIDER: S-EPMC8394144 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature