Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice.

ABSTRACT: Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonan

SUBMITTER: Lewandowska H 

PROVIDER: S-EPMC8395076 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature