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Evaluation of Interactions of Selected Olivacine Derivatives with DNA and Topoisomerase II.


ABSTRACT: Olivacine and ellipticine are model anticancer drugs acting as topoisomerase II inhibitors. Here, we present investigations performed on four olivacine derivatives in light of their antitumor activity. The aim of this study was to identify the best antitumor compound among the four tested olivacine derivatives. The study was performed using CCRF/CEM and MCF-7 cell lines. Comet assay, polarography, inhibition of topoisomerase II activity, histone acetylation, and molecular docking studies were performed. Each tested compound displayed interaction with DNA and topoisomerase II, but did not cause histone acetylation. Compound 2 (9-methoxy-5,6-dimethyl-1-({[1-hydroxy-2-(hydroxymethyl)butan-2-yl]amino}methyl)-6H-pyrido[4,3-b]carbazole) was found to be the best candidate as an anticancer drug because it had the highest affinity for topoisomerase II and caused the least genotoxic damage in cells.

SUBMITTER: Tylinska B 

PROVIDER: S-EPMC8395211 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Evaluation of Interactions of Selected Olivacine Derivatives with DNA and Topoisomerase II.

Tylińska Beata B   Dobosz Agnieszka A   Spychała Jan J   Cwynar-Zając Łucja Ł   Czyżnikowska Żaneta Ż   Kuźniarski Amadeusz A   Gębarowski Tomasz T  

International journal of molecular sciences 20210806 16


Olivacine and ellipticine are model anticancer drugs acting as topoisomerase II inhibitors. Here, we present investigations performed on four olivacine derivatives in light of their antitumor activity. The aim of this study was to identify the best antitumor compound among the four tested olivacine derivatives. The study was performed using CCRF/CEM and MCF-7 cell lines. Comet assay, polarography, inhibition of topoisomerase II activity, histone acetylation, and molecular docking studies were pe  ...[more]

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