Ngn3-Positive Cells Arise from Pancreatic Duct Cells.

ABSTRACT: The production of pancreatic β cells is the most challenging step for curing diabetes using next-generation treatments. Adult pancreatic endocrine cells are thought to be maintained by the self-duplication of differentiated cells, and pancreatic endocrine neogenesis can only be observed when the tissue is severely damaged. Experimentally, this can be performed using a method named partial duct ligation (PDL). As the success rate of PDL surgery is low because of difficulties in identifying the pancreatic duct, we previously proposed a method for fluorescently labeling the duct in live animals. Using this method, we performed PDL on neurogenin3 (Ngn3)-GFP transgenic mice to determine the origin of endocrine precursor cells and evaluate their potential to differentiate into multiple cell type

SUBMITTER: Kimura-Nakajima C 

PROVIDER: S-EPMC8395223 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature